Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

DOI:

10.3791/63875-v

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07:02 min

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April 15, 2022

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Rhiannon B. Werder*^1,2,3, Jessie Huang*^1,2, Kristine M. Abo², Olivia T. Hix², Kasey Minakin², Konstantinos-Dionysios Alysandratos², Carly Merritt², Kayleigh Berthiaume², Andrea B. Alber², Claire L. Burgess², Darrell N. Kotton*^1,2, Andrew A. Wilson*^1,2

¹Center for Regenerative Medicine,Boston University and Boston Medical Center, ²The Pulmonary Center and Department of Medicine,Boston University School of Medicine, ³QIMR Berghofer Medical Research Institute

Chapters

00:04Introduction
00:57Alveolosphere Dissociation
02:333D Plating of iAT2s
03:37Passaging of iAT2s to ALI
05:22Results: Human Induced Pluripotent StemCell-Derived Type 2 Alveolar Epithelial-Like Cells
06:05Conclusion

Summary

Automatic Translation

The present protocol describes human induced pluripotent stem cell-derived type 2 alveolar epithelial-like cells (iAT2s). These cells can be cultured as self-renewing spheres in 3D culture or adapted to air-liquid interface (ALI) culture.

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

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