Using Inducible Osteoblastic Lineage-Specific <em>Stat3</em> Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Yuanqi Liu; Siyuan Sun; Ziyi Jiang; Xinyi Gong; Yiling Yang; Yanfei Zhu; Hongyuan Xu; Anting Jin; Xiangru Huang; Xin Gao; Tingwei Lu; Jingyi Liu; Xinyu Wang; Qinggang Dai; Lingyong Jiang

doi:10.3791/65613

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Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Using Inducible Osteoblastic Lineage-Specific <em>Stat3</em> Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

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JoVE Journal Medicine

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

1,297 Views

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05:25 min

•

July 21, 2023

DOI:

10.3791/65613-v

DOI:

10.3791/65613-v

•

05:25 min

July 21, 2023

•

5 Views

Yuanqi Liu*^1,2,3,4,5, Siyuan Sun*^1,2,3,4,5, Ziyi Jiang*⁶, Xinyi Gong^1,2,3,4,5, Yiling Yang^1,2,3,4,5, Yanfei Zhu^1,2,3,4,5, Hongyuan Xu^1,2,3,4,5, Anting Jin^1,2,3,4,5, Xiangru Huang^1,2,3,4,5, Xin Gao^1,2,3,4,5, Tingwei Lu^1,2,3,4,5, Jingyi Liu^1,2,3,4,5, Xinyu Wang^1,2,3,4,5, Qinggang Dai^2,3,4,5,7, Lingyong Jiang^1,2,3,4,5

¹Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine, ²College of Stomatology,Shanghai Jiao Tong University, ³National Center for Stomatology, ⁴National Clinical Research Center for Oral Diseases, ⁵Shanghai Key Laboratory of Stomatology, ⁶Shanghai Starriver Bilingual School, ⁷The 2nd Dental Center, Ninth People’s Hospital, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine

Transcript

Our research focuses on the mechanism of bone remodeling under mechanical force, including alveolar bone and long bones. We try to answer how bones respond to mechanical stimuli to shed new light on skeletal mechanical biology for further clinical treatment. Currently, studies on OTM were limited to gene expression analysis in vivo or cellular function analysis in vitro.

To this end, a challenge arose. How to trace and explore the function of specific genes in specific lineages during OTM in vivo? That’s why we use inducible conditional knockout mice here.

Orthodontic tooth movement, which induces rampant bone remodeling, is a time-saving method to study the effects of mechanical force on bone remodeling compared to other models that have a long experimental period. Our protocol also reveals dynamic alveolar bone remodeling at different time points with inducible gene-specific knockout mice. There are many important cell lineages in the alveolar bone.

We aim to trace them and find out their underlying mechanisms during OTM. Furthermore, we will compare the mechanisms between alveolar bone and long bone remodeling to provide further clues for the potential of OTM model in biomechanical research.

Summary

This study provides a protocol for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describes methods for analyzing alveolar bone remodeling during orthodontic tooth movement, thus shedding light on skeletal mechanical biology.