Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

Fabian Dieter; Jendrik Grube; Tim Birkenhauer; Alice Quentin; Gunter P. Eckert

doi:10.3791/65730

Journal

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Immunology and Infection

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Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Cryopreservation and Bioenergetic Evaluation of Human Peripheral Blood Mononuclear Cells

1,126 Views

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04:10 min

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October 20, 2023

DOI:

10.3791/65730-v

DOI:

10.3791/65730-v

•

04:10 min

October 20, 2023

•

21 Views

Fabian Dieter¹, Jendrik Grube¹, Tim Birkenhauer¹, Alice Quentin¹, Gunter P. Eckert¹

¹Biomedical Research Center, Institute of Nutritional Sciences,Justus-Liebig-University of Giessen

Transcript

With the help of this method, we want to describe a procedure which is suitable to cryopreserve PBMCs in such a way that after thawing, the bioenergetic parameters are unchanged. One of the major experimental challenges of cryopreservation is that the freezing and thawing process can damage PBMCs, which affects the following bioenergetic measurements. With our protocol, we try to show a way to preserve PBMCs reliably without damage to the cells so that the measured bioenergetic parameters are not influenced.

The possibility of preserving PBMCs allows samples to be collected from different centers and measured at one location. It also makes measurement more time independent. Cryopreservation of PBMCs allows studies to be performed simultaneously at multiple sites where the cells are collected and preserved.

The collected samples can then be measured centrally. This streamlines the process and planning of studies.

Summary

Isolated peripheral blood mononuclear cells can be used for the analysis of immune functions and disorders, metabolic diseases, or mitochondrial functions. In this work, we describe a standardized method for the preparation of PBMCs from whole blood and the subsequent cryopreservation. Cryopreservation makes this time and place independent.