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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

DOI:
10.3791/50766-v

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09:01 min

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December 10, 2013

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, , , , , ,
1Laboratory of Pediatric Infectious Diseases, Department of Pediatrics,Radboud university medical center

Chapters

  • 00:05Title
  • 01:12Human Respiratory Syncytial Virus (RSV) A2 Culture
  • 02:39RSV Purification
  • 04:14Peripheral Blood Mononuclear Cell (PBMC) Isolation from Healthy Volunteer Blood
  • 05:50Stimulation of PBMC with Live RSV
  • 06:30Results: Representative Human PBMC Immune Responses to RSV Infection
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

Human respiratory syncytial virus (HRSV) can cause severe bronchiolitis in young infants. Part of the pathogenesis of severe HRSV disease is caused by the host immune response. Stimulation of primary human immune cells with HRSV provides a fast and reproducible model system to study activation of inflammatory pathways and infection.

Tags

Keywords: HRSVHuman Respiratory Syncytial VirusPBMCsPeripheral Blood Mononuclear CellsIn Vitro ModelImmune ResponseInnate ImmunityAdaptive ImmunityViral InfectionFlow CytometryTranscriptional ResponseTranslational Response

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