Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Isotta Sturniolo; Csongor V&#225;r&#243;czy; &#193;kos M&#225;t&#233; Bede; Csaba Heged&#369;s; M&#225;t&#233; &#193;. Dem&#233;ny; L&#225;szl&#243; Vir&#225;g

doi:10.3791/65922

Journal

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Cancer Research

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Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

JoVE Journal
Cancer Research
Author Produced

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JoVE Journal Cancer Research

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

2,336 Views

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13:19 min

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April 26, 2024

DOI:

10.3791/65922-v

DOI:

10.3791/65922-v

•

13:19 min

April 26, 2024

•

43 Views

Isotta Sturniolo^1,2, Csongor Váróczy^1,3, Ákos Máté Bede^1,3, Csaba Hegedűs¹, Máté Á. Demény^1,4, László Virág^1,4

¹Department of Medical Chemistry, Faculty of Medicine,University of Debrecen, ²Doctoral School of Molecular Medicine,University of Debrecen, ³National Academy of Scientist Education, ⁴HUN-REN-DE Cell Biology and Signaling Research Group

Summary

Here, we present a method to identify compounds that modulate the ADCC mechanism, an important cancer cell-killing mechanism of antitumor antibodies. The cytotoxic effect of NK cells is measured in breast cancer cell spheroids in the presence of Trastuzumab. Image analysis identifies live and dead killer and target cells in spheroids.