Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

We aim to understand how intrinsically disorder regions sense and respond to changes in the physical chemical properties of the environment. We combine biophysical, biochemical, genetic, and cell biology techniques to monitor how the structural ensemble of disorder regions change in living cells. Protein biophysics techniques are currently used to study the confirmation of IDRs.

These include nuclear magnetic resonance, circular dichroism, small angle x-ray scattering, and FRET. Most of these techniques can only be used in vitro. Studying the confirmation of IDRs in a cellular context is challenging with high cost and time consuming techniques.