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Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy

Article DOI: 10.3791/62490-v • 08:10 min • November 20th, 2021
November 20th, 2021 •
1, 1,2,3, 1, 1,4, 1, 5, 5, 1,2, 1,2,3
1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney

Summary
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A biomembrane force probe (BFP) is an in situ dynamic force spectroscopy (DFS) technique. BFP can be used to measure the spring constant of molecular interactions on living cells. This protocol presents spring constant analysis for molecular bonds detected by BFP.

Tags

Molecular Spring Constant Biomembrane Force Probe BFP Spectroscopy Single Molecule Binding Cell Membrane Mechanics Dynamic Force Spectroscopy Hooke's Law Red Blood Cell Mechanics Probe Micropipette Micro-pipette Aspirating Pressure
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