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Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy
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Bioengineering
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JoVE Journal Bioengineering
Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy

Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy

DOI:
10.3791/62490-v

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08:10 min

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November 20, 2021

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, , , , , , , ,
1School of Biomedical Engineering, Faculty of Engineering,The University of Sydney, 2Charles Perkins Centre,The University of Sydney, 3Heart Research Institute, 4Department of Chemistry,The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering,The University of Sydney

Summary

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A biomembrane force probe (BFP) is an in situ dynamic force spectroscopy (DFS) technique. BFP can be used to measure the spring constant of molecular interactions on living cells. This protocol presents spring constant analysis for molecular bonds detected by BFP.

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Keywords: Molecular Spring ConstantBiomembrane Force ProbeForce SpectroscopySingle Molecule BindingHooke’s LawRed Blood CellLigand ReceptorContact AreaMicropipetteForce-time DataApproachRetractCompressive PhaseTensile Phase

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