Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure
1.4K views
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05:34 min
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December 13th, 2024
DOI :10.3791/67100-v
December 13th, 2024
•1.4K views
Natascha Drude1 , Kai Diederich2 , Claudia U. Duerr3 , Nadine Haase4 , Christoph Harms5,6,7,8 , Frank Heppner9,10,11 , Marina Jendrach9,10,11 , Pia Kahnau2 , Marina Kolesnichenko12 , Lars Lewejohann2,13 , Claudia Kurreck1 , Anke Lohan14 , Marcus A. Mall15,16 , Dominik Müller4 , Stefan Nagel-Riedasch14 , Bastian Opitz17 , Laura Schaupp15,16 , Gilbert Schönfelder2,18 , Amelie Weber3 , Gerald Willimsky19,20,21 , Yalei Zang1,22 , Stephan P. Rosshart*23,24 , Andreas Diefenbach*3 , Stefan Jordan*3
1BIH QUEST Center for Responsible Research, Berlin Institute of Health (BIH) at Charité - Universitätsmedizin Berlin, 2German Federal Institute for Risk Assessment (BfR), German Centre for the Protection of Laboratory Animals (Bf3R), 3Institute of Microbiology, Infectious Diseases and Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 4Experimental and Clinical Research Center (ECRC) & Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft (MDC), 5Department of Experimental Neurology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Klinik und Hochschulambulanz für Neurologie, 6Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 7Einstein Center for Neuroscience, 8German Center for Cardiovascular Research (DZHK), partner site Berlin, 9Department of Neuropathology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10Cluster of Excellence, NeuroCure, 11German Center for Neurodegenerative Diseases (DZNE) Berlin, 12Department of Hepatology and Gastroenterology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 13Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, 14Forschungseinrichtungen für Experimentelle Medizin/FEM, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 15Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 16German Center for Lung Research (DZL), associated partner site, Berlin, 17Department of Infectious Diseases, Respiratory Medicine and Critical Care, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 18Division of Toxicology, Institute of Clinical Pharmacology and Toxicology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 19Institute of Immunology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 20German Cancer Research Center, 21German Cancer Consortium, partner site Berlin, 22Department of Radiology (including Pediatric Radiology), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 23Department of Microbiome Research, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 24Department of Medicine II, Faculty of Medicine, Medical Center - University of Freiburg
* These authors contributed equally
Here, we describe the structure and operating procedures, including microbial containment measures of a facility for "Wilding mice" using blood sampling for immunophenotyping as an example.
Chapters in this video
0:00
Introduction
2:12
Sampling Blood from Wildling Mice Maintained with Natural Microbiomes
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