February 17th, 2026
This protocol details a comprehensive method for protecting palatal donor sites by applying a collagen sponge and a mucoadhesive bandage, which is then stabilized with suspension sutures for enhanced, long-term wound healing.
This study focuses on protecting the palatal donor site and introduces a novel method to enhance its protection. Traditional methods for donor site management were either expensive or inefficient. This protocol provides long-term wound protection.
After marking the graft site, make a horizontal incision along the probe mark using a number 15 scalpel blade to a depth of 1.5 to 2.0 millimeters, extending about one millimeter beyond the intended graft length. Then make a vertical incision at the start point of the first horizontal incision to a depth of approximately 1.5 to 2.0 millimeters. Make another vertical incision at the endpoint of the first horizontal incision and extend the incision length approximately 0.5 millimeters beyond the desired gingival graft width.
At one end of the first horizontal incision, rotate the number 15 scalpel blade to an angle parallel to the bone surface. Gradually dissect the underlying connective tissue along the initial horizontal incision, advancing the blade approximately one millimeter beyond the intended graft width. Finally, make an inferior horizontal incision at the palatal donor site parallel to the first horizontal incision to a depth of approximately 1.5 to 2.0 millimeters.
Connect the two vertical incisions to free the gingival graft completely. After achieving initial homeostasis and preparing the wound bed, use sterile forceps to place the trimmed collagen sponge into the maxillary palatal donor site. Ensure the sponge is in close contact with the underlying connective tissue.
Then position the bandage on the surface of the collagen sponge and center it over the donor site. Ensure a uniform overlap of one to two millimeters onto the adjacent mucosa around the periphery. Lastly, use a 4-0 monofilament suture to place two horizontal mattress suspension sutures, one parallel and one cross around the same tooth or adjacent teeth using the same needle entry and exit points.
The mean total pain score decreased significantly from 3.71 at postoperative day one to 2.24 at day three, 0.69 at day seven and 0.19 at day 14. At the 14-day follow-up, the mean wound healing rate was 79.43%approximately, and the mean oral health impact Profile-14 score was 8.18. Future studies should generate stronger evidence-based clinical data to evaluate the protocol's efficiency, safety, and long-term outcomes.
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This article presents a novel protocol for protecting palatal donor sites following autologous soft tissue harvesting. The method combines a collagen sponge scaffold, a mucoadhesive bandage, and stabilizing suspension sutures to create a stable, long-lasting barrier that enhances wound healing, reduces postoperative pain, and improves patient comfort compared to traditional techniques.