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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
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Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

Article DOI: 10.3791/55064-v • 06:38 min • January 14th, 2017
January 14th, 2017 •
1, 1
1School of Plant Sciences, University of Arizona

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Summary
Automatic Translation

We describe a simple method for screening bacterial cultures for the production of compounds inhibitory towards other bacteria.

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Soft-agar Overlay Technique Screen Bacterially Produced Inhibitory Compounds Bacterial Strain Microbial Ecology Antagonistic Bacterial Interactions Inexpensive Easy To Perform Interpret Aretha Student Laboratory P Syringae King's Medium B Broth KB Broth Turbidity Mitomycin C Micro Centrifuge Sterilize Culture Supernatants Sodium Chloride One Molar Concentration Polyethylene Glycol (PEG 8000) Final Concentration
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