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Journal / Biology / ODELAY: A Large-scale Method for Multi-parameter…
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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Article DOI: 10.3791/55879-v • 11:19 min • July 3rd, 2017
July 3rd, 2017 •
1, 1,2, 1, 1,2
1Institute for Systems Biology, 2Center for Infectious Disease Research

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Summary
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We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.

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ODELAY Yeast Growth Multi-parameter Quantification High Throughput Microbiology Genetics Systems Biology Genetic Perturbations Drug Interactions Population Heterogeneity Agar Mold Assembly Acrylic Molds Ethanol Cleaning Forced Air Drying Bottom Pieces Upright Pieces Binder Clips Glass Slides Molten Agar Media
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