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Journal / Developmental Biology / Correlative Super-resolution and Electron…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Article DOI: 10.3791/56113-v • 12:28 min • November 10th, 2017
November 10th, 2017 •
1, 1, 1, 2, 1, 2, 1, 2,3, 1
1Center for Microscopy and Image Analysis, University of Zurich, 2Institute for Molecular Life Sciences, University of Zurich, 3Institute for Medical Genetics, University of Zurich

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Summary
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This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.

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Correlative Super-resolution Electron Microscopy Protein Localization Zebrafish Retina Microscopy Method Subcellular Organelles Larval Zebrafish Retinal Development Cellular Pathway Protein Mislocalization Mutants Technique Structural Context Tokuyasu Sections Silicone Wafers Platinum Shadowing Topography Larva Zebrafish Retina Studies Biological Systems Protein Expression Mouse Tissue
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