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Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

DOI:
10.3791/53635-v

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10:09 min

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May 25, 2016

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, , , ,
1Cell Biology,UMC Groningen, 2Clinical Genetics,Erasmus MC Rotterdam

Chapters

  • 00:05Title
  • 01:10Larvae Fixation and Embedding
  • 02:38Sectioning, Contrasting, and Mounting
  • 04:28Mounting the Sample in the Scanning Electron Microscope and Acquiring Images
  • 06:40Data Analysis
  • 07:33Results: Large Scale Scanning Transmission Electron Microscopy of Zebrafish Brain
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

Large-scale 2D electron microscopy (EM), or nanotomy, is the tissue-wide application of nanoscale resolution EM. Here we describe a universal method for nanotomy applied to investigate the zebrafish larval brain in health and upon non-invasive brain injury.

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Large-scale Scanning Transmission Electron MicroscopyNanotomyZebrafish BrainMacromolecular LevelDegenerating BrainNeurodegenerationType 1 Diabetes ResearchUnbiased InformationQuantitationOpen-access Data SharingImmune MaintenanceMicrogliaDisease ModelsCell CultureOverwhelming DataFixing Zebrafish LarvaeOsmium TetroxidePotassium FerrocyanideSodium CacodylateDouble Distilled WaterEthanol Embedding

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