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JoVE Journal
Biochemistry

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Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
 

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Article DOI: 10.3791/58461-v 06:45 min January 11th, 2019
January 11th, 2019

Chapters

Summary

Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.

Tags

Electron Paramagnetic Resonance EPR Spectroscopy Redox Signaling Reactive Oxygen Species Biologic Samples Spin Probes Experimental Design Cell Density Time-matched Controls Krebs-Henseleit Buffer DTPA Superoxide Dismutase CMH PMA
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