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Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Article DOI: 10.3791/58843-v • 08:51 min • November 19th, 2018
November 19th, 2018 •
1, 1,2, 1, 1,2, 1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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Summary
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A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through immunoprecipitation.

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Poly(pentafluorophenyl Acrylate) SiO2 Beads Protein Purification Biomolecule-activated Surfaces Drug Delivery Biological Target Detection Bio Separation Poly PFPA Brushes Amines Immobilization Of Antibodies Buffer Solution Few Hours Protein Purification Through Immunopurification Immobilized Antibody Target Antigens Silicon Dioxide Beads APTES Methanol Scintillation Vial Reaction Room Temperature Stirring Conical Tube Centrifuge Supernatant
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