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Biology

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Identification of protein complexes with quantitative proteomics in S. cerevisiae
 

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Article DOI: 10.3791/1225
March 4th, 2009

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Summary March 4th, 2009

Here we describe a new quantitative proteomics technique for identifying protein complexes in Saccharomyces cerevisiae. In this study, we have used the SILAC method together with affinity purification followed by tandem mass spectrometry to identify with high specificity the binding partners of an ER protein, Scs2p.

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