Articles by Siddhant Bhatia in JoVE

使用低温电子断层扫描和自动子断层扫描平均HIV包膜糖蛋白分子结构的测定

JoVE 2770 12/01/2011

Joel R. Meyerson^1,2, Tommi A. White¹, Donald Bliss³, Amy Moran³, Alberto Bartesaghi¹, Mario J. Borgnia¹, M. Jason V. de la Cruz¹, David Schauder¹, Lisa M. Hartnell¹, Rachna Nandwani^1,4, Moez Dawood⁵, Brianna Kim⁶, Jun Hong Kim⁷, John Sununu⁸, Lisa Yang⁹, Siddhant Bhatia¹⁰, Carolyn Subramaniam¹, Darrell E. Hurt¹¹, Laurent Gaudreault¹², Sriram Subramaniam¹

¹Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²The Medical Research Council Mitochondrial Biology Unit, University of Cambridge, ³National Library of Medicine, National Institutes of Health, ⁴Massachusetts Institute of Technology, ⁵William Fremd High School, ⁶University of Virginia, ⁷Duke University, ⁸Yale University, ⁹University of Notre Dame, ¹⁰Washington University in St. Louis, ¹¹Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, ¹²Thomas Jefferson High School for Science and Technology

协议描述了高通量的方法，以确定使用低温电子断层扫描和三维图像处理的膜蛋白的结构。它涵盖了样品制备的详细信息，数据采集，数据处理和解释，最后一个有代表性的目标的方法，HIV - 1包膜糖蛋白的生产。这些计算程序设计的方式，使研究人员和学生远程工作和作出贡献的数据处理和结构分析。