Histological Staining of Spinal Cord for Assessing EAE Disease Severity

February 23rd, 2024

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Carmen C. Ucciferri¹, Annette Gower², Nuria Alvarez-Sanchez^1,2, Heather Whetstone³, Valeria Ramaglia¹, Jennifer L. Gommerman¹, Koroboshka Brand-Arzamendi², Raphael Schneider^2,4, Shannon E Dunn^1,2,5

¹Department of Immunology, University of Toronto, ²Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, ³Sickkids Research Institute, The Hospital for Sick Children, ⁴BARLO MS Centre, St. Michael’s Hospital, ⁵Women’s College Research Institute, Women’s College Hospital

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Experimentele auto-immuun encefalomyelitis (EAE) dient als een diermodel van multiple sclerose. Dit artikel beschrijft een benadering voor het scoren van ruggenmergontsteking, demyelinisatie en axonaal letsel bij EAE. Daarnaast wordt een methode gepresenteerd om oplosbare neurofilamentlichtniveaus in het muizenserum te kwantificeren, waardoor de beoordeling van axonaal letsel bij levende muizen wordt vergemakkelijkt.

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Histological Staining of Spinal Cord for Assessing EAE Disease Severity

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