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Biology
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在原位议定书“蝴蝶蛹使用Riboprobes翼
JoVE Journal
Biology
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JoVE Journal
Biology
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
Please note that all translations are automatically generated.
Click here for the English version.
在原位议定书“蝴蝶蛹使用Riboprobes翼
DOI:
10.3791/208-v
•
06:19 min
•
May 28, 2007
•
Diane Ramos
,
Antonia Monteiro
1
Department of Biological Sciences
,
SUNY-University at Buffalo
,
2
Dept. Ecology and Evolutionary Biology
,
Yale University
Chapters
00:09
Collecting pre-pupae
00:36
Dissecting and fixing wings
01:08
Pre-hybridization
02:27
Making hybridization buffer
03:09
Hybridization
03:59
Applying antibody
04:31
Detection and development
05:35
Mounting
05:59
Result: patterns of Engrailed
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了检验在Bicyclus anynana蛹右翼组织的基因表达,我们目前在使用riboprobes原位杂交优化协议。我们还提供指引,为进一步优化本议定书在其他鳞翅目物种蛹翅膀。
Tags
In Situ Protocol
Butterfly Pupal Wings
Riboprobes
In Situ Hybridizations
Gene Expression
Transcription
Developmental Biology
Spatial Patterns
Temporal Patterns
Antibodies
Protein Products
Larval Wing Discs
Fragile Pupal Wings
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