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Journal / Biology / 异种移植模型中循环猪特异性DNA的定量…
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异种移植模型中循环猪特异性DNA的定量
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异种移植模型中循环猪特异性DNA的定量

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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Summary
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在该协议中，设计了猪特异性底剂，构建了含质粒的猪特异性DNA片段，并建立了定量标准曲线。使用物种特异性底法，cpsDNA在猪对小鼠细胞移植模型和猪对猴子动脉贴片移植模型中通过qPCR进行量化。

Tags

生物学， 问题 163， 特定底像， 循环猪特异性 DNA， qPCR， 异种移植， 生物标志物， 免疫排斥
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