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Kvantifisering av sirkulerende grisespesifikt DNA i blodet av en Xenotransplantasjonsmodell
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Kvantifisering av sirkulerende grisespesifikt DNA i blodet av en Xenotransplantasjonsmodell

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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I denne protokollen ble svinespesifikke primere designet, plasmider-inneholdende svinspesifikke DNA-fragmenter ble konstruert, og standardkurver for kvantitet ble etablert. Ved hjelp av artsspesifikke primere ble cpsDNA kvantifisert av qPCR i celletransplantasjonsmodeller for gris-til-mus og gris-til-ape arterie patch transplantasjon modeller.

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Biologi utgave 163 spesifikke primere sirkulerende grisespesifikt DNA qPCR xenotransplantasjon biomarkør immunavvisning
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