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Kvantifiering av cirkulerande Grisspecifikt DNA i blodet hos en Xenotransplantation-modell
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Kvantifiering av cirkulerande Grisspecifikt DNA i blodet hos en Xenotransplantation-modell

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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I detta protokoll utformades porcin specifika primers, plasmider-innehållande svin specifika DNA-fragment konstruerades, och standard kurvor för kvantisering fastställdes. Med hjälp av artspecifika primers, var cpsDNA kvantifieras av qPCR i gris-till-mus cell transplantation modeller och gris-till-apa artär patch transplantation modeller.

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Biologi specifika grund- och klövströ cirkulerande grisspecifikt DNA qPCR xenotransplantation biomarkör immunavstötning
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