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JoVE Journal Biochemistry
Complementazione dell'attività di splicing da parte di un complesso snRNP Galectin-3 - U1 su perline
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Complementazione dell'attività di splicing da parte di un complesso snRNP Galectin-3 - U1 su perline

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

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Questo articolo descrive le procedure sperimentali per (a) l'esaurimento di U1 snRNP da estratti nucleari, con concomitante perdita di attività di splicing; e (b) ricostituzione dell'attività di splicing nell'estratto impoverito di U1 da particelle di galectina-3 - U1 snRNP legate a perline accoppiate covalentemente con anticorpi anti-galectina-3.

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Biochimica splicing pre-mRNA spliceosoma saggio di splicing senza cellule frazionamento del gradiente di glicerolo complesso U1 snRNP galectine
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