Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Komplementering af splejsning aktivitet af en…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
Komplementering af splejsning aktivitet af en Galectin-3 - U1 snRNP Kompleks på perler
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Journal (Biochemistry)
Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
Journal (Biochemistry)
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
 
Click here for the English version

Komplementering af splejsning aktivitet af en Galectin-3 - U1 snRNP Kompleks på perler

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

I denne artikel beskrives forsøgsprocedurerne for a) udtømning af U1 snRNP fra nukleare ekstrakter med deraf følgende tab af splejsning; og b) rekonstitution af splejsning aktivitet i U1-udtømte ekstrakt af galectin-3 - U1 snRNP partikler bundet til perler kovalent kombineret med anti-galectin-3 antistoffer.

Tags

Biokemi Udgave 166 præ-mRNA splejsning splejsning cellefri splejsning assay glycerol gradient fraktionering U1 snRNP kompleks galectiner
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter