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Journal / Biochemistry / 구슬에 갈렉틴-3 - U1 snRNP 복합체에 의한 접합 활동의…
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Biochemistry

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JoVE Journal Biochemistry
구슬에 갈렉틴-3 - U1 snRNP 복합체에 의한 접합 활동의 보완
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구슬에 갈렉틴-3 - U1 snRNP 복합체에 의한 접합 활동의 보완

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

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이 문서에서는 (a) 핵 추출물에서 U1 snRNP의 고갈에 대한 실험 절차에 대해 설명하며, 접합 활동의 수반되는 손실; (b) Galectin-3에 의한 U1 고갈 된 추출물에서 접합 활성의 재구성 - U1 snRNP 입자는 항 갈류 -3 항체와 함께 공동으로 구슬에 결합된다.

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생화학 문제 166 전 mRNA 접합 화려한 세포 없는 접합 분석 글리세롤 그라데이션 분획 U1 snRNP 복합체 galectins
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