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JoVE Journal Biochemistry
Aanvulling van splicingactiviteit door een Galectin-3 - U1 snRNP-complex op kralen
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Aanvulling van splicingactiviteit door een Galectin-3 - U1 snRNP-complex op kralen

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

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Dit artikel beschrijft de experimentele procedures voor (a) depletie van U1 snRNP uit nucleaire extracten, met gelijktijdig verlies van splicingactiviteit; en (b) reconstitutie van splicingactiviteit in het U1-uitgeputte extract door galectine-3 - U1 snRNP-deeltjes gebonden aan kralen covalent gekoppeld aan anti-galectine-3-antilichamen.

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Biochemie pre-mRNA splicing spliceosoom celvrije splicing assay glycerol gradiënt fractionering U1 snRNP complex galectines
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