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Journal / Developmental Biology / Robust differentiering af humane iPSC'er i en ren …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Robust differentiering af humane iPSC'er i en ren population af adipocytter for at studere adipocytassocierede lidelser
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Robust differentiering af humane iPSC'er i en ren population af adipocytter for at studere adipocytassocierede lidelser

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

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Protokollen tillader generering af en ren adipocytpopulation fra inducerede pluripotente stamceller (iPSC'er). Retinsyre bruges til at differentiere iPSC'er til mesenkymale stamceller (MSC'er), der anvendes til fremstilling af adipocytter. Derefter anvendes en sorteringsmetode baseret på Nilrød farvning til at opnå rene adipocytter.

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Denne måned i JoVE udgave 180 inducerede pluripotente stamceller mesenkymale stamceller adipocytdifferentiering cellesortering
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