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Journal / Developmental Biology / Robust differentiering av humana iPSC till en ren …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Robust differentiering av humana iPSC till en ren population av adipocyter för att studera adipocytassocierade störningar
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Robust differentiering av humana iPSC till en ren population av adipocyter för att studera adipocytassocierade störningar

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

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Protokollet möjliggör generering av en ren adipocytpopulation från inducerade pluripotenta stamceller (iPSC). Retinsyra används för att differentiera iPSC till mesenkymala stamceller (MSC) som används för att producera adipocyter. Därefter används en sorteringsmetod baserad på Nile röd färgning för att erhålla rena adipocyter.

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Denna månad i JoVE nummer 180 inducerade pluripotenta stamceller mesenkymala stamceller adipocytdifferentiering cellsortering
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