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Journal / Developmental Biology / Robust differensiering av humane iPSCs til en ren …
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Developmental Biology

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JoVE Journal Developmental Biology
Robust differensiering av humane iPSCs til en ren populasjon av adipocytter for å studere adipocytt-assosierte lidelser
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Robust differensiering av humane iPSCs til en ren populasjon av adipocytter for å studere adipocytt-assosierte lidelser

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

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Protokollen tillater generering av en ren adipocytpopulasjon fra induserte pluripotente stamceller (iPSCs). Retinsyre brukes til å differensiere iPSCs til mesenkymale stamceller (MSC) som brukes til å produsere adipocytter. Deretter brukes en sorteringsmetode basert på Nilrødfarging for å oppnå rene adipocytter.

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Denne måneden i JoVE indusert pluripotente stamceller mesenkymale stamceller adipocytt differensiering cellesortering
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