Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Diferenciación robusta de iPSCs humanas en una…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
Diferenciación robusta de iPSCs humanas en una población pura de adipocitos para estudiar trastornos asociados a adipocitos
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
Journal (Developmental Biology)
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Journal (Developmental Biology)
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
 
Click here for the English version

Diferenciación robusta de iPSCs humanas en una población pura de adipocitos para estudiar trastornos asociados a adipocitos

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

El protocolo permite la generación de una población de adipocitos puros a partir de células madre pluripotentes inducidas (iPSCs). El ácido retinoico se utiliza para diferenciar las iPSC en células madre mesenquimales (MSC) que se utilizan para producir adipocitos. Luego, se utiliza un enfoque de clasificación basado en la tinción de rojo del Nilo para obtener adipocitos puros.

Tags

Este mes en JoVE Número 180 indujo células madre pluripotentes células madre mesenquimales diferenciación de adipocitos clasificación celular
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter