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Journal / Immunology and Infection / 伯氏疏螺旋体（Borrelia）伯氏疏螺旋体外转录测定的基本成分…
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Immunology and Infection

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JoVE Journal Immunology and Infection
伯氏疏螺旋体（Borrelia）伯氏疏螺旋体外转录测定的基本成分
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伯氏疏螺旋体（Borrelia）伯氏疏螺旋体外转录测定的基本成分

Article DOI: 10.3791/64134-v • 07:15 min • July 22nd, 2022
July 22nd, 2022 •
1, 1, 1
1School of Medicine, Creighton University

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体外转录测定可以破译伯氏疏螺旋体的转录调控机制。该协议描述了纯化伯氏疏螺旋体RNA聚合酶并进行体外转录反应的步骤。使用体外转录测定的实验方法需要对活性RNA聚合酶进行可靠的纯化和储存。

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本月在JoVE上，第185期， 疏螺旋体，疏 螺旋体， 体外 转录，RNA聚合酶，RpoD，RpoC
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