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JoVE Journal Immunology and Infection
Composants essentiels des tests de transcription in vitro de Borreliella (Borrelia) burgdorferi
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Composants essentiels des tests de transcription in vitro de Borreliella (Borrelia) burgdorferi

Article DOI: 10.3791/64134-v • 07:15 min • July 22nd, 2022
July 22nd, 2022 •
1, 1, 1
1School of Medicine, Creighton University

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Les tests de transcription in vitro peuvent déchiffrer les mécanismes de régulation transcriptionnelle chez Borreliella burgdorferi. Ce protocole décrit les étapes pour purifier l’ARN polymérase de B. burgdorferi et effectuer des réactions de transcription in vitro. Les approches expérimentales utilisant des tests de transcription in vitro nécessitent une purification et un stockage fiables de l’ARN polymérase active.

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Ce mois-ci dans JoVE numéro 185 Borrelia Borreliella transcription in vitro ARN polymérase RpoD RpoC
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