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Immunology and Infection

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JoVE Journal Immunology and Infection
Componentes essenciais de ensaios de transcrição in vitro de Borreliella (Borrelia) burgdorferi
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Componentes essenciais de ensaios de transcrição in vitro de Borreliella (Borrelia) burgdorferi

Article DOI: 10.3791/64134-v • 07:15 min • July 22nd, 2022
July 22nd, 2022 •
1, 1, 1
1School of Medicine, Creighton University

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Ensaios de transcrição in vitro podem decifrar os mecanismos de regulação transcricional em Borreliella burgdorferi. Este protocolo descreve as etapas para purificar a RNA polimerase de B. burgdorferi e realizar reações de transcrição in vitro. Abordagens experimentais usando ensaios de transcrição in vitro requerem purificação e armazenamento confiáveis da RNA polimerase ativa.

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Este mês em JoVE Edição 185 Borrelia Borreliella transcrição in vitro RNA polimerase RpoD RpoC
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