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Journal / Immunology and Infection / Wesentliche Bestandteile von Borreliella…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Wesentliche Bestandteile von Borreliella (Borrelia) burgdorferi In-vitro-Transkriptionsassays
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Wesentliche Bestandteile von Borreliella (Borrelia) burgdorferi In-vitro-Transkriptionsassays

Article DOI: 10.3791/64134-v • 07:15 min • July 22nd, 2022
July 22nd, 2022 •
1, 1, 1
1School of Medicine, Creighton University

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In-vitro-Transkriptionsassays können die Mechanismen der Transkriptionsregulation in Borreliella burgdorferi entschlüsseln. Dieses Protokoll beschreibt die Schritte zur Aufreinigung der B. burgdorferi RNA-Polymerase und zur Durchführung von In-vitro-Transkriptionsreaktionen. Experimentelle Ansätze mit In-vitro-Transkriptionsassays erfordern eine zuverlässige Aufreinigung und Lagerung der aktiven RNA-Polymerase.

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Diesen Monat in JoVE Ausgabe 185 Borrelien Borreliella in vitro Transkription RNA-Polymerase RpoD RpoC
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