JoVE   
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Biology

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Neuroscience

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Immunology and Infection

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Clinical and Translational Medicine

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Bioengineering

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Applied Physics

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Chemistry

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Behavior

  
You do not have subscription access to articles in this section. Learn more about access.

  JoVE Environment

|   

JoVE Science Education

General Laboratory Techniques

You do not have subscription access to videos in this collection. Learn more about access.

Basic Methods in Cellular and Molecular Biology

You do not have subscription access to videos in this collection. Learn more about access.

Model Organisms I

You do not have subscription access to videos in this collection. Learn more about access.

Model Organisms II

You have trial access to videos in this collection until May 31, 2014.

Automatic Translation

This translation into Spanish was automatically generated through Google Translate.
English Version | Other Languages

 JoVE Clinical and Translational Medicine

En vivo doble sustrato bioluminiscentes imagen

1, 1, 1, 1

1Case Comprehensive Cancer Center, Case Western Reserve University

Article
    Downloads Comments Metrics
     

    Summary

    Cite this Article

    Wendt, M. K., Molter, J., Flask, C. A., Schiemann, W. P. In vivo Dual Substrate Bioluminescent Imaging. J. Vis. Exp. (56), e3245, doi:10.3791/3245 (2011).

    Disclosures

    Materials

    Name Company Catalog Number Comments
    pGL4.2-Puro [luc2/Puro] Vector Promega Corp. E6751
    Glomax Mult-idection system Promega Corp.
    IVIS 200 series Caliper Life Sciences
    Dual luciferase reporter Assay system Promega Corp. E1960
    Rediject c–lenterazine-h Caliper Life Sciences 760506
    D-Luciferin K-Salt Caliper Life Sciences 122796

    References

    1. Wetterwald, A., et al. Optical imaging of cancer metastasis to bone marrow: a mouse model of minimal residual disease. Am. J. Pathol. 160, 1143-1153 (2002).
    2. Wendt, M.K., Drury, L.J., Vongsa, R.A., & Dwinell, M.B. Constitutive CXCL12 Expression Induces Anoikis in Colorectal Carcinoma Cells. Gastroenterology. 135, 508 (2008).
    3. Wendt, M. & Schiemann, W. Therapeutic targeting of the focal adhesion complex prevents oncogenic TGF-beta signaling and metastasis. Breast Cancer Research. 11, R68 (2009).
    4. Korpal, M., et al. Imaging transforming growth factor-beta signaling dynamics and therapeutic response in breast cancer bone metastasis. Nat Med. (2009).
    5. Giampieri, S., et al. Localized and reversible TGFbeta signalling switches breast cancer cells from cohesive to single cell motility. Nat Cell Biol. 11, 1287-1296 (2009).
    6. Giampieri, S., Pinner, S., & Sahai, E. Intravital imaging illuminates transforming growth factor beta signaling switches during metastasis. Cancer Res. 70, 3435-3439 (2010).
    7. DeNardo, D.G., et al. CD4(+) T cells regulate pulmonary metastasis of mammary carcinomas by enhancing protumor properties of macrophages. Cancer Cell. 16, 91-102 (2009).
    8. Wyckoff, J. et al. A Paracrine Loop between Tumor Cells and Macrophages Is Required for Tumor Cell Migration in Mammary Tumors. Cancer Res. 64, 7022-7029 (2004).
    9. Kim, M.Y., et al. Tumor self-seeding by circulating cancer cells. Cell. 139, 1315-1326 (2009).
    10. Aslakson, C.J. & Miller, F.R. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res. 52, 1399-1405 (1992).
    11. Wendt, M.K., Smith, J.A., & Schiemann, W.P. Transforming growth factor-beta-induced epithelial-mesenchymal transition facilitates epidermal growth factor-dependent breast cancer progression. Oncogene. 29, 6485-6498 (2010).
    12. Hawkins, E., Unch, J., Murhpy, N., Vidugiriene, J., Scurria, M., Klaubert, D., & Wood, K. Bright Light, No Lysis. Measuring Renilla Luciferase Luminescence in Living Cells. http://www.promega.com/pnotes/90/12727_10/12727_10.pdf (2005).
    13. Roncali, E., et al. New device for real-time bioluminescence imaging in moving rodents. J. Biomed. Opt. 13, 054035 (2008).

    Comments

    0 Comments

    Post a Question / Comment / Request

    You must be signed in to post a comment. Please or create an account.

    Metrics

    Waiting
    simple hit counter