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Chapters
- 00:05Title
- 02:11Growth of M. tuberculosis Biofilms for Drug Resistance
- 03:39Determining the Frequency of Drug Tolerant Persisters in M. tuberculosis Biofilms
- 06:39Culture Morphology and Drug Resistance
- 08:23Conclusion
Mycobacterium tuberculosis forms drug tolerant biofilms when cultured in certain conditions. Here we describe methods for culturing M. tuberculosis biofilms and determining the frequency of drug tolerant persisters. These protocols will be useful for further studies into the mechanisms of drug tolerance in M. tuberculosis.
Tags
Mycobacterium TuberculosisGrowthBiofilmsStress ToleranceAntibioticsSurvivalMolecular MechanismsPathogenSelf-assemblingSurface AttachedMatrix Encapsulated StructuresBiofilm GrowthCommunitiesGenetic ComponentsSurface AttachmentIntercellular CommunicationsExtracellular Polymeric Substances (EPS)Environmental Stress TolerancePhysiological AdaptationBacilliComplex ArchitectureAnti-tuberculosis DrugsIsoniazidRifampicin
