Primary Adipose Culture
Primary Adipose Culture - Related Videos
08:43
Primary Neuronal Cultures
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2023
The complexity of the brain often requires neuroscientists to use a simpler system for experimental manipulations and observations. One powerful approach is to generate a primary culture by dissecting nervous system tissue, dissociating it into single cells, and growing those cells in vitro. Primary cultures make neurons and glia easily accessible to the experimental tools required for techniques like genetic manipulation and time-lapse imaging. Furthermore, these cultures represent a highly...
08:14
Using Primary Neurosphere Cultures to Study Primary Cilia
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Cited by 3 •
2017
The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.
08:20
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
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Cited by 75 •
2016
The vascular endothelial cells play a significant role in many important cardiovascular disorders. This article describes a simple method to isolate and expand endothelial cells from the mouse aorta without using any special equipment. Our protocol provides an effective means of identifying mechanisms in endothelial cell physiopathology.
03:52
Isolating Adipose-Derived Stem Cells from Murine Periaortic Adipose Tissue
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2025
This video demonstrates a technique for isolating and culturing fluorescently labeled neural crest-derived adipose-derived stem cells (ADSCs) from murine periaortic arch adipose tissue. Following isolation, the NCADSCs are detected, sorted using fluorescence-activated cell sorting, and subsequently cultured on a plate, promoting cell proliferation.
07:35
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
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Cited by 16 •
2015
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
FAQs
Enzymatic dissociation separates mature adipocytes from the stromal vascular fraction, creating distinct cellular populations for controlled study. This separation allows researchers to examine whether observed responses arise from mature fat-storing cells or from associated stromal and vascular components. The resulting comparison can clarify how different adipose cell populations contribute to tissue-level regulation and communication.
Cells obtained directly from fat retain donor-specific features that may be lost during long-term cell-line maintenance. Consequently, their responses to hormones, nutrients, inflammatory signals, and other environmental changes can reflect characteristics of the original tissue more closely. This makes the system useful when biological variation among donors is relevant to interpreting adipose-cell behavior.
Defined culture conditions determine whether the resulting cells can attach, remain viable, or undergo differentiation. These outcomes are important because the same isolated material can support different experimental goals depending on how it is maintained. Researchers therefore select conditions according to whether they want to observe cellular maintenance, differentiation-related changes, or responses to controlled environmental signals.
Changes observed in cultured adipose cells can be interpreted in relation to lipid storage, metabolic regulation, and communication with neighboring cells. For behavior-focused research, this creates a bridge between a cellular response and a broader tissue function. For example, altered responses to hormones or nutrients may help explain how adipose tissue adjusts its activity under changing environmental conditions.
A typical workflow begins with obtaining adipose cells or tissue directly from fat, followed by enzymatic dissociation to separate mature adipocytes from the stromal vascular fraction. The resulting cells are then maintained in defined culture conditions selected to support attachment, viability, or differentiation. Researchers can subsequently expose the cultures to controlled hormones, nutrients, or inflammatory signals and examine their responses.
This approach is useful when researchers need to test how adipose cells respond to a specific environmental change while limiting the complexity of the intact organism. Controlled exposure to hormones, nutrients, or inflammatory signals can reveal cellular effects that relate to lipid storage, metabolic regulation, or signaling between neighboring cells. Donor-specific characteristics add further context to those findings.