Microfluidic Platform

A microfluidic platform is an integrated system that manipulates very small volumes of fluids through networks of microscale channels, making biological experiments more controlled, efficient, and resource sparing. At these dimensions, fluids generally move in laminar flow, while channel geometry, valves, pumps, and diffusion govern mixing, separation, and the formation of precise chemical or cellular gradients. In biological techniques, microfluidic platforms support cell culture, single-cell analysis, particle sorting, molecular diagnostics, and automated assays using small sample volumes. Their ability to integrate multiple steps on one device improves reproducibility and enables rapid, high-throughput studies of dynamic cellular and biochemical processes.

Microfluidic Platform - Related Videos

Research

JoVE Journal - Bioengineering
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Fluorescence detection methods for microfluidic droplet platforms

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Cited by 20 •

2011

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

Research

JoVE Journal - Bioengineering

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

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Cited by 20 •

2014

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

High-throughput Protein Expression Generator Using a Microfluidic Platform

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Cited by 22 •

2012

We present a microfluidic approach for the expression of protein arrays. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for experimental use.

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

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Cited by 40 •

2013

Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This protocol provides detailed steps on how to use the system for a broad range of applications.

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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Cited by 8 •

2017

We demonstrate a microfluidic platform with an integrated surface electrode network that combines resistive pulse sensing (RPS) with code division multiple access (CDMA), to multiplex detection and sizing of particles in multiple microfluidic channels.

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