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High-throughput Protein Expression Generator Using a Microfluidic Platform
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Chapters
- 00:05Title
- 01:26Device Fabrication
- 03:27DNA Arraying and Device Alignment
- 04:42Priming and Activating the Device
- 06:29Surface Chemistry
- 07:50Protein Expression
- 08:35Results: Microfluidic Device Fabrication
- 09:02Conclusion
We present a microfluidic approach for the expression of protein arrays. The device consists of thousands of reaction chambers controlled by micro-mechanical valves. The microfluidic device is mated to a microarray-printed gene library. These genes are then transcribed and translated on-chip, resulting in a protein array ready for experimental use.
Tags
High-throughput Protein Expression GeneratorMicrofluidic PlatformSystems BiologyHigh-throughput Screening ExperimentsBiochemical AssaysBiophysical AssaysCell-based AssaysMicrofluidic Large-scale IntegrationMicromechanical ValvesIn Vitro ExpressionProtein ArraysPING (Protein Interaction Network Generator)Protein-protein InteractionsProtein-RNA InteractionsProtein-DNA InteractionsReaction ChambersMicroarrayerMicroarray Spotting Techniques
