Afm Probe
Afm Probe - Related Videos
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
08:21
Quantitative Hardness Measurement by Instrumented AFM-indentation
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Cited by 6 •
2016
This experimental protocol describes how atomic force microscopy can be used to measure hardness at the true nanometer scale and to detect single atomistic plasticity events.
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
08:31
Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope (AFM-SECM)
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Cited by 2 •
2021
Atomic force microscopy (AFM) combined with scanning electrochemical microscopy (SECM), namely, AFM-SECM, can be used to simultaneously acquire high-resolution topographical and electrochemical information on material surfaces at nanoscale. Such information is critical to understanding heterogeneous properties (e.g., reactivity, defects, and reaction sites) on local surfaces of nanomaterials, electrodes and biomaterials.
08:29
Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
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Cited by 12 •
2016
To obtain basic information on the sorption and recycling of gold from aqueous systems the interaction of Au(III) and Au(0) nanoparticles on S-layer proteins were investigated. The sorption of protein polymers was investigated by ICP-MS and that of proteinaceous monolayers by QCM-D. Subsequent AFM enables the imaging of the nanostructures.
FAQs
During scanning, short-range forces between the tip and specimen bend the flexible cantilever. A laser detects this deflection, allowing the system to translate changes in cantilever position into information about surface topography or mechanical behavior. This force-based readout enables measurements from biological specimens without requiring the sample to conduct electricity.
The cantilever must respond to the short-range forces acting between the probe tip and the specimen. Its bending provides the signal used for measurement, while the nanoscale tip interacts with surfaces such as membranes or biomolecules. This arrangement supports investigation of soft biological structures through either surface mapping or mechanical measurements.
Force spectroscopy uses interactions between the probe and specimen to assess properties that a surface image alone cannot provide. The resulting measurements can indicate stiffness, adhesion, and molecular interactions. In biology, these outputs help characterize soft samples and investigate how their mechanical or interaction-related behavior differs across cellular or biomolecular structures.
A typical measurement places the sharp probe over the specimen, scans the surface, and monitors cantilever bending with a laser. The recorded signal is then used to generate topographic or mechanical data, depending on the measurement approach. This workflow can be applied to cell membranes, biomolecules, and other soft biological samples.
The overview supports using AFM probes with biological samples in either air or liquid. The appropriate environment depends on the specimen and the measurement context, since cells, membranes, biomolecules, and other soft materials may be examined under different conditions. Working in these environments broadens the range of biological surfaces that can be investigated.
Measurements from AFM probes support studies of cellular structure, biomaterials, and disease-related changes. Imaging can show features of cell membranes and biomolecules, while force spectroscopy adds information about stiffness, adhesion, and molecular interactions. Together, these outcomes help researchers relate nanoscale surface or mechanical behavior to biological structure and condition.