Antisense Rna
Antisense Rna - Related Videos
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
10:30
Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy
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Cited by 23 •
2016
Exon skipping is currently a most promising therapeutic option for Duchenne muscular dystrophy (DMD). To expand the applicability for DMD patients and to optimize the stability/function of the resulting truncated dystrophin proteins, a multi-exon skipping approach using cocktail antisense oligonucleotides was developed and we demonstrated systemic dystrophin rescue in a dog model.
06:21
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
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Cited by 20 •
2013
A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Cited by 22 •
2013
Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.
11:09
Chromatin Isolation by RNA Purification (ChIRP)
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Cited by 380 •
2012
ChIRP is a novel and rapid technique to map genomic binding sites of long noncoding RNAs (lncRNAs). The method takes advantage of the specificity of anti-sense tiling oligonucleotides to allow the enumeration of lncRNA-bound genomic sites.
FAQs
The regulatory outcome depends on which step of RNA handling is affected. Pairing can obstruct ribosome access and reduce translation, change messenger RNA stability, promote degradation, or influence RNA processing and transcription. These alternatives allow antisense RNA to control gene expression through several mechanisms rather than producing one uniform cellular response.
Cellular context determines which regulatory pathway becomes dominant after antisense RNA interacts with its target. In one setting, the major effect may be reduced ribosome access; in another, altered stability, degradation, processing, or transcription may prevail. This context dependence is important when interpreting experiments across bacteria, plants, and animals.
Antisense RNA can influence multiple stages of gene regulation, including translation, messenger RNA stability, RNA degradation, processing, and transcription. Its effects therefore extend beyond simply reducing protein production. Examining which stage changes helps connect an observed expression difference to the underlying regulatory mechanism and clarifies how post-transcriptional control operates.
Researchers can design antisense RNA molecules as targeted experimental tools to regulate expression of a selected gene through its RNA. Observing the resulting change helps investigate that gene's regulation and the cellular consequences of altering its RNA. This approach is especially useful for examining post-transcriptional control without treating all gene regulation as a single process.
Experiments can reveal whether regulation is associated with blocked ribosome access, changed RNA stability, degradation, altered processing, or transcriptional effects. These outcomes provide evidence about how a gene is controlled at the RNA level. They also help explain broader biological phenomena, including microbial adaptation and disease-related gene regulation.
Antisense RNA research supports gene-silencing strategies and the development of targeted treatments for disease-related gene regulation. It also provides a framework for studying naturally occurring regulation in bacteria, plants, and animals, including mechanisms linked to microbial adaptation. Together, these applications connect basic RNA biology with experimental and therapeutic approaches.