Clathrin Triskelion
Clathrin Triskelion - Related Videos
17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
0 Views •
Cited by 8 •
2011
Clathrin-mediated endocytosis depends on adaptor proteins that coordinate cargo selection and clathrin coat assembly. Here we describe procedures to study adaptor-clathrin physical interaction and live cell imaging approaches using as a model the yeast endocytic adaptor protein Sla1p.
01:12
Clathrin Coated Vesicles
0 Views •
2023
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
03:28
Labeling Murine T Helper Lymphocytes using Radiolabeled Monoclonal Antibodies
0 Views •
2025
In this video, we demonstrate the intracellular radiolabeling of T helper cells using radioactive isotope-conjugated monoclonal antibodies for non-invasive positron emission tomography imaging.
12:40
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
0 Views •
Cited by 6 •
2014
Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.
09:27
An Introduction to Endocytosis and Exocytosis
0 Views •
2023
Cells can take in substances from the extracellular environment by endocytosis and actively release molecules into it by exocytosis. Such processes involve lipid membrane-bound sacs called vesicles. Knowledge of the molecular architecture and mechanisms of both is key to understanding normal cell physiology, as well as the disease states that arise when they become defective. This video will first briefly review a few pivotal discoveries in the history of endo- and exocytosis research. Next,...
FAQs
The three flexible legs of each clathrin triskelion interact with legs from neighboring units, producing a connected polyhedral lattice. This organization gives the coat structural stability while imposing curvature on the membrane. As more triskelia join the lattice, the growing scaffold helps shape a membrane bud that can participate in vesicle formation.
Three clathrin heavy chains form the main structural framework of a triskelion, while associated light chains are part of the assembled unit. The heavy-chain arrangement provides the legs and supports lattice formation through interactions with neighboring triskelia. Together, these components create the coat architecture required to organize membrane regions during trafficking.
Adaptor proteins provide the connection between the clathrin coat, selected cargo, and membrane lipids. This bridging function helps concentrate particular receptors, nutrients, or signaling molecules at the membrane region where a coat forms. Consequently, clathrin assembly is linked not only to membrane curvature but also to the selective loading of cellular material.
Assembly connects molecular structure with the movement of material between cellular compartments. A triskelion lattice can organize membrane deformation, while adaptor proteins couple that scaffold to cargo and lipids. Studying these coordinated events clarifies how trafficking occurs from the plasma membrane and trans-Golgi network and how membrane organization supports intracellular transport.
This system is especially relevant to clathrin-mediated endocytosis and trafficking from the plasma membrane and trans-Golgi network. Researchers can use it to investigate how cells internalize receptors, nutrients, and signaling molecules, then relate coat organization to the movement of those materials. The topic therefore connects vesicle formation with broader questions about cellular communication and compartmental organization.
Investigating altered triskelion assembly can reveal how defects in coat formation affect membrane organization and vesicle trafficking. Such studies also help connect molecular changes with diseases caused by disrupted intracellular transport. Comparing normal and impaired assembly provides a framework for determining which structural interactions are important for cargo movement and cellular function.