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Chapters
- 00:05Title
- 01:21Expression of Flourescently Tagged CME Components in Cultured Cells
- 02:42Imaging Clathrin-coated Pit (CCP) and Cargo Dynamics Using TIRFM
- 05:22Analysis of Endocytic Dynamics by Manual Verification
- 06:32Analysis of Endocytic Dynamics by Objective Recognition
- 10:40Results: Anlysis of the Endocytic Dynamics of the μ‐opioid‐receptor (MOR), a GPCR, and its Endocytic Adaptor Protein β‐arrestin
- 12:18Conclusion
Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.
