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12:40 min
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October 20, 2014
DOI:
Summary
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Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.
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Cite this Article
Soohoo, A. L., Bowersox, S. L., Puthenveedu, M. A. Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy. J. Vis. Exp. (92), e51805, doi:10.3791/51805 (2014).
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