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January 26, 2011
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Clathrin-mediated endocytosis depends on adaptor proteins that coordinate cargo selection and clathrin coat assembly. Here we describe procedures to study adaptor-clathrin physical interaction and live cell imaging approaches using as a model the yeast endocytic adaptor protein Sla1p.
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Feliciano, D., Bultema, J. J., Ambrosio, A. L., Di Pietro, S. M. In vivo and in vitro Studies of Adaptor-clathrin Interaction. J. Vis. Exp. (47), e2352, doi:10.3791/2352 (2011).
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