Click Chemistry Labeling
Click Chemistry Labeling - Related Videos
06:46
Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry
0 Views •
Cited by 11 •
2011
A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
10:54
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
0 Views •
Cited by 7 •
2017
Here, we describe a protocol for radiolabeling and in vivo testing of tridentate 99mTc(I) chelate-tetrazine derivatives for pre-targeting and bioorthogonal chemistry.
10:40
Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
0 Views •
Cited by 8 •
2018
BLISS, a dual labeling protocol for studying lignification dynamics, was developed. Using synthetic monolignol reporters and a sequential combination of SPAAC and CuAAC bioorthogonal click reactions, this methodology paves the way to in-depth analysis of the factors that regulate the biogenesis of lignins in planta.
12:31
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
0 Views •
Cited by 14 •
2012
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
08:42
Coordination Chemistry Complexes
0 Views •
2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
FAQs
Selective, high-yield reactions allow a chemical tag to be attached mainly where an azide or alkyne handle has been introduced. Because the coupling can occur under mild, aqueous conditions, researchers can label proteins, glycans, lipids, or nucleic acids while limiting disruption to cellular or biochemical systems. This selectivity supports clearer localization and tracking results.
Copper-catalyzed azide-alkyne cycloaddition uses copper to promote linkage between azide and alkyne groups, whereas strain-promoted cycloaddition avoids copper. Both connect the introduced chemical handle to a detectable probe, but the copper-free approach is especially relevant when labeling living systems where mild conditions and reduced disruption are important.
Azide and alkyne groups serve as chemical handles that provide a defined connection point for a fluorescent dye, biotin, or another detectable tag. Researchers can introduce these handles into proteins, glycans, lipids, or nucleic acids before performing the coupling reaction. The selected handle and reaction determine how the biomolecule becomes detectable or recoverable.
Fluorescent dyes make labeled biomolecules observable, supporting studies of where molecules occur in cells, tissues, or biochemical samples. Biotin provides a different detection strategy that can support biomolecule enrichment. Other detectable tags may be selected for tracking or analysis, so the probe should match whether the experiment emphasizes localization, recovery, or molecular movement.
A typical workflow first introduces an azide or alkyne group into the biomolecule of interest, then exposes that handle to a complementary probe under suitable reaction conditions. The probe may carry fluorescence, biotin, or another detectable tag. After coupling, the labeled material can be visualized, enriched, or tracked in cells, tissues, or biochemical samples.
This approach is useful when researchers need to follow specific biomolecules without substantially disturbing the surrounding biological system. In biology, it supports investigations of molecular localization, protein synthesis, cellular metabolism, and biomolecular interactions. Its flexibility across cellular, tissue, and biochemical samples allows the same general strategy to address both spatial and molecular questions.