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JoVE Journal
Chemistry

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Visualizing Lignification Dynamics in Plants with Click Chemistry
 

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Article DOI: 10.3791/56947-v 10:40 min January 26th, 2018
January 26th, 2018

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Summary

BLISS, a dual labeling protocol for studying lignification dynamics, was developed. Using synthetic monolignol reporters and a sequential combination of SPAAC and CuAAC bioorthogonal click reactions, this methodology paves the way to in-depth analysis of the factors that regulate the biogenesis of lignins in planta.

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