Dual Optical Mapping
Dual Optical Mapping - Related Videos
09:24
Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
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Cited by 30 •
2011
This article describes the basic procedures for conducting optical mapping experiments in the Langendorff-perfused rabbit heart using the panoramic imaging system, and the dual (voltage and calcium) imaging modality.
08:25
Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
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Cited by 9 •
2015
This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.
08:13
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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Cited by 71 •
2011
This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.
11:48
Optical Mapping of Langendorff-perfused Rat Hearts
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Cited by 16 •
2009
This article describes a high temporal and spatial resolution technique to optically image action potential movement on the surface of Langendorff-perfused rat hearts using a potentiometric dye (di-8-ANEPPS).
11:07
High-resolution Optical Mapping of the Mouse Sino-atrial Node
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Cited by 17 •
2016
Here, we present a protocol for optical mapping of electrical activity from the mouse right atrium and especially the sino-atrial node, at a high spatial and temporal resolution.
FAQs
The key analytical step is aligning voltage and calcium signals in both space and time. This reveals whether electrical activation is followed by an appropriate calcium release and recovery pattern across cardiac tissue. The resulting relationship provides a tissue-level view of excitation-contraction coupling, rather than treating electrical and calcium activity as unrelated measurements.
Separate voltage-sensitive and calcium-sensitive indicators allow each physiological signal to be measured through its own optical channel. This separation helps distinguish changes in membrane voltage from changes in intracellular calcium while preserving simultaneous observation. Researchers can therefore compare the timing and distribution of the two signals without relying on either measurement alone.
High-speed cameras capture rapid changes in both signals, making it possible to examine conduction, repolarization, calcium release, and calcium recovery across living tissue. Comparing these time-dependent patterns can expose abnormal activity that might be missed when only one signal is recorded. The approach is especially useful for examining coordinated or disrupted cardiac behavior.
A typical workflow uses living cardiac tissue, applies voltage-sensitive and calcium-sensitive fluorescent indicators, and records the resulting signals through separate optical channels. High-speed cameras collect the activity, after which the voltage and calcium data are aligned spatially and temporally. Researchers then evaluate conduction, repolarization, calcium dynamics, and their relationship.
Researchers choose the dual approach when they need to assess how electrical activity relates to intracellular calcium behavior. A voltage-only or calcium-only measurement cannot fully characterize both sides of excitation-contraction coupling. Simultaneous recording supports more complete analysis of tissue responses, including coordinated activation, recovery, and abnormalities associated with arrhythmogenic activity.
In cardiovascular research, the technique supports studies of cardiac electrophysiology, disease mechanisms, drug effects, and tissue-level responses. Its value comes from connecting electrical behavior with calcium dynamics across living cardiac tissue. This combined information can help investigators evaluate how interventions or disease-related changes affect conduction, repolarization, calcium handling, and abnormal activity.