White Preadipocytes
White Preadipocytes - Related Videos
09:00
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
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Cited by 19 •
2021
This report describes a protocol for the simultaneous isolation of primary brown and white preadipocytes from newborn mice. Isolated cells can be grown in culture and induced to differentiate into fully mature white and brown adipocytes. The method enables genetic, molecular, and functional characterization of primary fat cells in culture.
05:30
Exogenous Mitochondrial Transfer in Differentiating Brown Adipocytes and AGPAT2-Deficient Preadipocytes
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2026
Exogenous mitochondria of human and murine origin can be internalized by differentiating preadipocytes and tracked throughout adipogenic differentiation using imaging and genetic tools. While these mitochondria physically interact with the endogenous mitochondrial network, they do not increase the expression of markers associated with mature brown adipocytes.
08:30
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
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Cited by 6 •
2015
Here, we present a protocol for encapsulation of catabolic cells, which consume lipids for heat production in intra-abdominal adipose tissue and increase energy dissipation in obese mice.
06:32
Isolation of Preadipocytes from Broiler Chick Embryos
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Cited by 1 •
2022
The present protocol describes a simple method for isolating preadipocytes from adipose tissue in broiler embryos. This method enables isolation with high yield, primary culture, and adipogenic differentiation of preadipocytes. Oil Red O staining and lipid/DNA stain measured the adipogenic ability of isolated cells induced with differentiation media.
08:36
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
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Cited by 21 •
2016
Here we present methodology for the production of a focal stroke in murine white matter by local injection of an irreversible endothelial nitric oxide synthase (eNOS) inhibitor (L-Nio). Presented are two stereotactic variations, retrograde neuronal tracing, and fresh tissue labeling and dissection that expand the potential applications of this technique.
FAQs
Insulin and glucocorticoids act as initiating signals that change gene expression in white preadipocytes. These changes activate transcriptional regulators, including PPARγ and C/EBP, which establish the program required for lipid accumulation and mature adipocyte function. Studying this signaling sequence helps researchers determine how external cues control the progression of fat-cell development.
PPARγ and C/EBP function as transcription factors that coordinate the gene-expression changes accompanying adipogenesis. Their activation links early differentiation signals to later cellular outcomes, including lipid storage and adipocyte activity. Measuring changes associated with these regulators allows investigators to evaluate whether white preadipocytes are advancing toward a mature functional state.
The transition connects developmental changes in gene expression with metabolic behavior. As cells differentiate, they accumulate lipid and acquire functions associated with energy storage and endocrine regulation. Examining both stages allows researchers to distinguish effects on cell development from effects on mature adipocyte activity, which is important when interpreting metabolism-related experiments.
Researchers culture the precursor cells under controlled conditions, expose them to signals that promote differentiation, and assess resulting changes in gene expression, lipid accumulation, or metabolic activity. This workflow provides a defined system for comparing treated and untreated cells. It can reveal how specific compounds or signaling conditions alter the development of fat-cell characteristics.
Experiments can examine whether cells undergo adipogenesis, accumulate lipid droplets, change expression of differentiation-related genes, or develop metabolic activity associated with mature adipocytes. These readouts provide complementary information: gene expression indicates regulatory changes, whereas lipid accumulation and cellular activity show associated functional outcomes. Together, they help characterize how experimental conditions influence adipose-cell development.
These cultured cells provide a controlled way to investigate processes involved in fat-cell development and metabolic regulation. Researchers can examine how differentiation or altered metabolic activity relates to conditions such as obesity and insulin resistance. The model also supports testing compounds that may increase or reduce adipogenesis or modify cellular metabolic responses.