Embryo Ventricle Targeting
Embryo Ventricle Targeting - Related Videos
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
04:17
Long-term Orthotopic Transplantation of Tumor Cells into Zebrafish Embryos: An Efficient Method to Deliver Tumor Cells into the Fourth Ventricle of Zebrafish Embryos for Long-term Analysis of Tumor Cell Behavior
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2025
This video demonstrates the orthotopic transplantation of zebrafish brain tumor cells into the fourth ventricle of an immunocompetent zebrafish embryo. This procedure allows for the long-term study of tumor cell invasion and behavior.
06:17
Manual Drainage of the Zebrafish Embryonic Brain Ventricles
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Cited by 9 •
2012
We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.
10:04
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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Cited by 9 •
2013
How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.
10:17
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
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Cited by 9 •
2016
We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.
FAQs
Placement within the ventricular cavity positions the introduced reagent next to the neuroepithelium, the developing tissue that surrounds the cavity. This proximity allows the material to contact nearby neural cells during early development. Consequently, researchers can examine how a localized genetic, labeling, or gene-expression manipulation affects neural tissue without treating the entire embryo in the same way.
The neuroepithelium provides the immediate tissue interface for materials placed in the ventricular cavity. Because it contains developing neural cells, contact with this layer connects the delivery site to processes such as tissue patterning and cell fate. Observing responses in this region helps investigators relate the introduced material to early nervous-system formation.
Spatial control restricts delivery to a defined region of the developing nervous system. This focus helps researchers associate an introduced construct, label, or regulatory reagent with nearby neural tissues and their developmental responses. The approach therefore supports more precise studies of morphogenesis, gene-expression effects, and cell populations than an approach that does not control the delivery location.
A typical workflow uses a fine micropipette or comparable delivery tool, positions its tip at the embryonic ventricular cavity, and places the selected reagent into that space. The delivered material then contacts the surrounding neuroepithelium and developing neural cells. Researchers subsequently examine labeling, gene-expression changes, tissue patterning, or cell-fate outcomes relevant to the experiment.
The essential components are the material selected for the experiment and a fine micropipette or comparable delivery tool capable of placing it in the ventricular cavity. Materials may include genetic constructs, labeling reagents, or agents intended to manipulate gene expression. Their value depends on reaching the targeted cavity and contacting the surrounding neural tissue.
Researchers use this approach when they need access to the embryonic nervous system during early development. Applications include introducing genetic constructs, labeling specific cell populations, manipulating gene expression, and examining tissue patterning or cell fate. These experiments can clarify cellular mechanisms involved in neurodevelopment, morphogenesis, and the formation of neural tissues.