Fitc-dextran
Fitc-dextran - Related Videos
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Cited by 10 •
2018
Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
12:00
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Cited by 9 •
2015
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
07:35
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Cited by 8 •
2011
This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.
FAQs
Molecular weight determines how readily the tracer moves through a barrier, so different FITC-dextran variants probe different transport ranges. Smaller or larger dextran forms can therefore reveal distinct changes in membrane or epithelial permeability. Selecting the appropriate size helps researchers relate fluorescence to structural integrity rather than treating every tracer signal as equivalent.
The fluorescein label supplies the measurable optical signal: after excitation at an appropriate wavelength, it emits green light. Researchers can capture this emission by fluorescence microscopy for spatial information or measure it with a plate-based assay for quantitative comparison. Thus, the readout can emphasize localization or overall signal, depending on the experimental format.
The biological question determines the interpretation: movement across a membrane or epithelial layer reflects permeability, whereas fluorescence associated with cells can support studies of endocytosis and intracellular trafficking. FITC-dextran therefore links a measured signal to either barrier passage or cellular handling, provided the experiment distinguishes the relevant location and process.
A practical workflow begins by selecting a molecular-weight variant that matches the barrier or transport question. The tracer is then examined in the chosen biological system, and fluorescence is collected either by microscopy or a plate-based assay. Researchers compare the resulting signal with the experimental conditions to quantify permeability, transport, or uptake in a consistent format.
Applications include intestinal barrier studies, blood-brain barrier models, and investigations of vascular leakage. The tracer can also support experiments examining endocytosis or intracellular trafficking. These uses make it relevant to both tissue-level barrier biology and cell-level transport research, while molecular-weight selection helps tailor the measurement to the system being studied.
Differences in fluorescence between experimental conditions can provide quantitative evidence of altered permeability, transport, or uptake. Researchers can use these comparisons to assess structural integrity and examine changes associated with disease, drugs, or other experimental treatments. The result is a measurable readout that connects biological conditions with changes in barrier or cellular behavior.